Salhab A S, Nooh N G, Dujovne C A
Anesth Analg. 1978 Nov-Dec;57(6):605-9.
The effects of volatile anesthetics were assessed in freshly isolated rat hepatocytes by surface-scanning electron microscopy and by measuring leakage of cellular enzymes, lactate dehydrogenase and beta-glucuronidase into the surrounding medium. The order of potency in regard to their capacity to produce alterations of these parameters was halothane = methoxyflurane greater than ether = control. The extent of enzyme leakage from hepatocytes exposed to halothane or methoxyflurane was both dose dependent and, for the first 30 minutes, time dependent. Surface scanning of the isolated hepatocytes showed that both halothane and methoxyflurane produced enzyme leakage and morphologic changes in cellular membranes, but ether did not. These studies demonstrate that scanning electron microscopy and enzyme leakage from cells are useful for the evaluation of drug-induced changes in lever cells in vitro. The relation between these drug-induced changes and clinical hepatotoxicity remains to be elucidated.
通过表面扫描电子显微镜以及测量细胞内酶（乳酸脱氢酶和β-葡萄糖醛酸酶）向周围培养基中的泄漏情况，对新鲜分离的大鼠肝细胞中挥发性麻醉剂的作用进行了评估。就产生这些参数改变的能力而言，其效力顺序为：氟烷 = 甲氧氟烷 ＞ 乙醚 = 对照。暴露于氟烷或甲氧氟烷的肝细胞中酶的泄漏程度既呈剂量依赖性，且在最初30分钟内也呈时间依赖性。对分离的肝细胞进行表面扫描显示，氟烷和甲氧氟烷均可导致酶泄漏及细胞膜形态改变，但乙醚不会。这些研究表明，扫描电子显微镜以及细胞内酶泄漏对于体外评估药物诱导的肝细胞变化很有用。这些药物诱导的变化与临床肝毒性之间的关系仍有待阐明。
