Iversen P H, Clausen O P, Iversen U M, Rohrbach R
Cell Tissue Kinet. 1976 Jan;9(1):77-97. doi: 10.1111/j.1365-2184.1976.tb01255.x.
Hairless mice were given 2 mg Bleomycin i.p. in 1-0 ml saline on two successive days. By a stathmokinetic method, by micro-flow fluorometry and by autoradiography certain kinetic parameters were measured during 10 days after the last injection. Cell counts were made and the turnover time of the differentiating cells estimated. Protein synthesis was estimated by the uptake of radioactive histidine, and dry cell mass measured by weighing. Bleomycin affected cell proliferation in the epidermis by depressing biphasically both the number of cells in, and the passage of cells through, the cell cycle phases: S, G2 and M, most probably by directly affecting late G1 cells and cells in mitosis. The time between the two minima of depressed DNA synthesis corresponded to the mean generation time of the basal cells. Histidine uptake and dry cell mass were slightly affected, but the turnover time of the differentiating cells was prolonged. Bleomycin thus had a strong long-lasting inhibitory effect on epidermal cell proliferation and a marked inhibitory effect on epidermal cell maturation in mice.
给无毛小鼠连续两天腹腔注射2毫克博来霉素（溶于1.0毫升生理盐水中）。通过静止动力学方法、微流荧光测定法和放射自显影术，在最后一次注射后的10天内测量了某些动力学参数。进行了细胞计数并估算了分化细胞的周转时间。通过放射性组氨酸的摄取估算蛋白质合成，并通过称重测量干细胞质量。博来霉素通过双相抑制细胞周期各期（S期、G2期和M期）的细胞数量及细胞通过这些时期的过程，影响表皮细胞增殖，最有可能是直接影响G1晚期细胞和有丝分裂期细胞。DNA合成抑制的两个最低点之间的时间对应于基底细胞的平均世代时间。组氨酸摄取和干细胞质量受到轻微影响，但分化细胞的周转时间延长。因此，博来霉素对小鼠表皮细胞增殖具有强烈的持久抑制作用，对表皮细胞成熟具有显著抑制作用。
