Fritsch P O, Pohlin G, Längle U, Elias P M
J Invest Dermatol. 1981 Sep;77(3):287-91. doi: 10.1111/1523-1747.ep12482459.
Hairless albino mice were fed aromatic retinoid in daily doses ranging from 1 to 50 mg/kg body weight. In animals receiving 1, 5, and 10 mg/kg, the thickness of the epidermis (vertical epidermal cross sectional areas) and the 3H-thymidine labeling indices were determined throughout a 3-week observation period. The following additional parameters were determined for animals fed 5 mg/kg; individual and cumulative cross sectional areas of epidermal cell nuclei and cytoplasms, nuclear-cytoplasm ratios, mean epidermal cell volume and mean number of epidermal cells. In animals fed 5 mg/kg for 7 days, an analysis of the cell kinetic parameters was performed including determination of duration of S and G2 phases, mean generation time and the proportion of noncycling (Go) cells. The changes induced by the aromatic retinoid were found to be dose dependent and consisted of a transitory cellular hypertrophy and a persistent numerical hyperplasia linked to increased labeling indices. The mean basal cell generation time was found to be greatly accelerated owing to a shortening of all cell cycle phases investigated.
给无毛白化小鼠每日喂食芳香维甲酸，剂量范围为1至50毫克/千克体重。在接受1、5和10毫克/千克剂量的动物中，在整个3周的观察期内测定表皮厚度（垂直表皮横截面积）和3H-胸腺嘧啶核苷标记指数。对于喂食5毫克/千克的动物，还测定了以下参数：表皮细胞核和细胞质的个体和累积横截面积、核质比、平均表皮细胞体积和平均表皮细胞数量。在喂食5毫克/千克7天的动物中，进行了细胞动力学参数分析，包括测定S期和G2期的持续时间、平均世代时间和非循环（Go）细胞的比例。发现芳香维甲酸诱导的变化具有剂量依赖性，包括短暂的细胞肥大和与标记指数增加相关的持续性细胞数量增生。由于所研究的所有细胞周期阶段均缩短，发现平均基底细胞世代时间大大加快。
