Ahmad F, Bigelow C C
Can J Biochem. 1978 Nov;56(11):1003-5. doi: 10.1139/o78-158.
The isothermal denaturation of ribonuclease A by mixed denaturant systems was investigated at 25 degrees C. It was observed that low concentrations of lithium chloride stabilize the protein against denaturation by urea, even though the salt itself is a denaturant. This study also provides, for the first time, the most convincing evidence that the lithium chloride denatured ribonuclease A contains some of the native secondary and tertiary structure.
在25摄氏度下研究了混合变性剂体系对核糖核酸酶A的等温变性。观察到，即使氯化锂本身是一种变性剂，但低浓度的氯化锂能稳定蛋白质使其不被尿素变性。该研究还首次提供了最有说服力的证据，即氯化锂变性的核糖核酸酶A含有一些天然的二级和三级结构。
