Gupta S L, Talwar G P
Biochem J. 1968 Dec;110(3):401-6. doi: 10.1042/bj1100401.
Growth hormone stimulates the synthesis of RNA in hypophysectomized rat liver. The question whether the hormonal stimulation of RNA synthesis is due to the activation of repressed cistrons or to other factors was studied. Nuclear RNA from the livers of adult female hypophysectomized and growth-hormone-treated rats was examined for molecular homology by hybridization techniques: no new species of RNA were detected after hormone treatment. The template activity of the chromatin for RNA synthesis is also not increased by the action of growth hormone. Short- and long-pulse-labelling experiments demonstrate that the hormonal stimulation of RNA synthesis is most marked in experiments where the period of incorporation of radioactive precursors is limited to 1-2hr. It is concluded that the hormone influences essentially the rate of RNA synthesis in these tissues.
生长激素可刺激垂体切除大鼠肝脏中RNA的合成。研究了RNA合成的激素刺激是由于被抑制顺反子的激活还是其他因素所致的问题。通过杂交技术检测成年雌性垂体切除及生长激素处理大鼠肝脏的核RNA的分子同源性：激素处理后未检测到新的RNA种类。生长激素的作用也未增加染色质对RNA合成的模板活性。短期和长期脉冲标记实验表明，在放射性前体掺入期限于1 - 2小时的实验中，RNA合成的激素刺激最为明显。得出的结论是，激素主要影响这些组织中RNA合成的速率。
