氯离子及其他一价无机阴离子对天冬氨酸氨基转移酶可见吸收光谱的影响。
The influence of chloride and other univalent inorganic anions on the visible-absorption spectrum of aspartate aminotransferase.
作者信息
Bergami M, Marino G, Scardi V
出版信息
Biochem J. 1968 Dec;110(3):471-3. doi: 10.1042/bj1100471.
Abstract
- The influence of Cl(-), Br(-), NO(3) (-) and F(-) ions on the visible-absorption spectrum of deionized aspartate aminotransferase was investigated. 2. Except for F(-), these anions caused an increase of the extinction at 430mmu with a concomitant decrease of that at 362mmu. 3. The affinity constants for Cl(-) and NO(3) (-) ions were calculated by a procedure based on the assumption that the anion stabilizes the protonated form of the enzyme chromophore (lambda(max.) 430mmu). 4. The true pK of the chromophore of the enzyme was found to be 5.25.
摘要
- 研究了Cl(-)、Br(-)、NO(3) (-)和F(-)离子对去离子化天冬氨酸转氨酶可见吸收光谱的影响。2. 除F(-)外，这些阴离子导致430nm处吸光度增加，同时362nm处吸光度降低。3. Cl(-)和NO(3) (-)离子的亲和常数是通过一种基于阴离子稳定酶发色团质子化形式（最大吸收波长430nm）这一假设的方法计算得出的。4. 发现该酶发色团的真实pK值为5.25。
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本文引用的文献
1
Glutamic aspartic transaminase. I. Assay, purification, and general properties.谷氨酸天冬氨酸转氨酶。I. 测定、纯化及一般性质。
J Biol Chem. 1959 Jan;234(1):51-7.
2
Purification and general properties of aspartate aminotransferase of ox heart.牛心脏天冬氨酸氨基转移酶的纯化及一般性质
Biochem J. 1966 Jun;99(3):589-94. doi: 10.1042/bj0990589.
3
[Purification of aspartate aminotransferase from the bovine heart].[从牛心脏中纯化天冬氨酸氨基转移酶]
Boll Soc Ital Biol Sper. 1965 Apr 30;41(8):438-40.
4
Isolation and characterization of multiple forms of glutamate-asparate aminotransferase from pig heart.猪心脏中多种形式谷氨酸-天冬氨酸转氨酶的分离与鉴定
J Biol Chem. 1967 May 25;242(10):2397-409.
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