小球藻异柠檬酸裂解酶受中间代谢产物的抑制作用
The inhibition, by intermediary metabolites, of isocitrate lyase from Chlorella pyrenoidosa.
作者信息
John P C, Syrett P J
出版信息
Biochem J. 1968 Dec;110(3):481-4. doi: 10.1042/bj1100481.
Abstract
- Intermediary metabolites, arising from the glyoxylate by-pass, were tested for their effect on the activity of isocitrate lyase, the first enzyme of the by-pass. 2. Oxaloacetate and pyruvate inhibited the enzyme when present at concentrations similar to that of the substrate. 3. Inhibition was competitive and did not depend on a non-optimum pH. The affinities determined at the pH optimum were: K(i) (oxaloacetate), 3.7x10(-5)m; K(i) (pyruvate), 6.6x10(-5)m. 4. The significance of the inhibitions is discussed with particular reference to the known inhibition by phosphoenolpyruvate.
摘要
- 对由乙醛酸支路产生的中间代谢产物进行了测试，以考察它们对该支路的第一种酶——异柠檬酸裂解酶活性的影响。2. 当草酰乙酸和丙酮酸的浓度与底物浓度相似时，它们会抑制该酶。3. 这种抑制是竞争性的，且不依赖于非最适pH。在最适pH下测定的亲和力为：K(i)（草酰乙酸），3.7×10⁻⁵m；K(i)（丙酮酸），6.6×10⁻⁵m。4. 特别参照已知的磷酸烯醇丙酮酸抑制作用，对这些抑制作用的意义进行了讨论。
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