Nusbickel F R, Swartz W J
Histochemistry. 1978 Dec 13;58(4):339-41. doi: 10.1007/BF00495391.
The non-specific artifactual precipitate found in glycol methacrylate (GMA) embedded tissues, following acid phosphatase histochemical incubation, can now be eliminated with brief ultrasonication. A treatment of 1 to 2 min totally eliminated the artifactual residue, leaving the true histochemical reaction product and the physical integrity of the tissues intact. With this technique, most loosely bound precipitates, which clutter and mask GMA or conventionally embedded tissues, may now be treated and eliminated.
在酸性磷酸酶组织化学孵育后，在甲基丙烯酸乙二醇酯（GMA）包埋组织中发现的非特异性人为沉淀，现在可以通过短暂超声处理消除。1至2分钟的处理完全消除了人为残留物，使真正的组织化学反应产物和组织的物理完整性保持完好。通过这种技术，现在可以处理和消除大多数松散结合的沉淀物，这些沉淀物会使GMA或传统包埋组织变得杂乱并掩盖其结构。
