Miese H, Müller W E, Wollert U
Arch Int Pharmacodyn Ther. 1978 Nov;236(1):18-26.
The binding of sulfamethoxypyridazine to human and bovine serum albumin was determined at eight constant molar drug/albumin ratios for seven different albumin concentrations ranging from 0.1% to 1.5%. The serum albumin concentration affects the determination of the albumin binding of the drug in two different ways. 1. The relative affinity of the albumins for the drug increases with increasing albumin concentrations, while the numbers of binding sites remain constant. 2. The association constants taken from Scatchard plots decrease with increasing albumin concentrations. It is concluded that a direct comparison of binding constants obtained with different albumin concentrations, as widely done, can lead to misinterpretations and should be avoided.
在药物/白蛋白摩尔比恒定的八个水平下，测定了磺胺甲氧嗪与人血清白蛋白及牛血清白蛋白的结合情况，白蛋白浓度范围为0.1%至1.5%，共七种不同浓度。血清白蛋白浓度以两种不同方式影响药物与白蛋白结合的测定。1. 白蛋白对药物的相对亲和力随白蛋白浓度增加而增加，而结合位点数量保持不变。2. 根据Scatchard图得出的缔合常数随白蛋白浓度增加而降低。得出的结论是，像广泛进行的那样，直接比较不同白蛋白浓度下获得的结合常数可能会导致误解，应该避免。
