Guerrero-Munoz F, Cerreta K V, Guerrero M L, Way E L
J Pharmacol Exp Ther. 1979 Apr;209(1):132-6.
The effect of morphine on the uptake of 45Ca++ was studied in synaptosomes from mouse brain using two procedures, centrifugation and filtration. The addition of morphine (1.7 x 10(-7) or 3.4 x 10(-7) M) reduced 45CA++ uptake by either technique, although the basal 45Ca++ uptake by the filtration method was approximately 7-fold higher than that by the centrifugation procedure. Similar effects were obtained after acute morphine treatment with 10 mg/kg s.c. Previous naloxone in vitro treatment (1.9 x 10(-8) M) or in vivo administration (2 mg/kg s.c.) reversed the morphine inhibition of the 45Ca++ uptake. On the other hand, after the animal was rendered tolerant and dependent by morphine pellet implantation, an enhancement of the synaptosomal 45Ca++ uptake was observed. It is concluded that changes in Ca++ fluxes in synaptosomes observed after acute and chronic morphine treatment may be involved with morphine pharmacological action related with analgesia, tolerance and physical dependence.
采用离心和过滤两种方法，研究了吗啡对小鼠脑突触体摄取45Ca++的影响。添加吗啡（1.7×10(-7)或3.4×10(-7) M）会降低两种技术下45Ca++的摄取量，不过通过过滤法测得的基础45Ca++摄取量比离心法高约7倍。皮下注射10 mg/kg吗啡进行急性处理后也得到了类似结果。之前在体外（1.9×10(-8) M）或体内（皮下注射2 mg/kg）给予纳洛酮可逆转吗啡对45Ca++摄取的抑制作用。另一方面，通过植入吗啡丸使动物产生耐受性和依赖性后，观察到突触体45Ca++摄取增强。得出的结论是，急性和慢性吗啡处理后观察到的突触体Ca++通量变化可能与吗啡的镇痛、耐受和身体依赖等药理作用有关。
