Straube G, Gerullis C, Blumenau R, Fritsche W
Zentralbl Bakteriol Naturwiss. 1978;133(7-8):698-705.
The incorporation of glycine-2-14C and adenine-U-14C in cell material and riboflavin was investigated in order to determine the proportions metabolites were channeled in growth processes and product synthesis of Eremothecium ashbyii. Extraction- and measurement methods were developed to compare the incorporation of radioactive metabolites into different cell fractions. Young cells incorporate the labeled compounds in a high rate into the cell material. With increasing age of the culture the incorporation of radioactivity is more directed to riboflavin. 96 hours old cells incorporate about seven times more 14C-adenine into riboflavin than into the cell material.
为了确定代谢产物在阿舒假囊酵母生长过程和产物合成中的分配比例，研究了甘氨酸-2-¹⁴C和腺嘌呤-U-¹⁴C掺入细胞物质和核黄素的情况。开发了提取和测量方法以比较放射性代谢产物掺入不同细胞组分的情况。年轻细胞将标记化合物快速掺入细胞物质中。随着培养时间延长，放射性掺入更多地指向核黄素。96小时龄的细胞将¹⁴C-腺嘌呤掺入核黄素的量比掺入细胞物质的量多约7倍。
