Bronson F H, Vom Saal F S
Endocrinology. 1979 May;104(5):1247-55. doi: 10.1210/endo-104-5-1247.
The regulation of the preovulatory release of LH by steroids was examined in the mouse, a species in which ovulation is strongly influenced by priming pheromones. Ovariectomized mice were implanted with estradiol in Silastic capsules to involve negative feedback. Preovulatory-like LH surges then were induced by injections of either estradiol benzoate (EB) or progesterone. LH surges were not observed in the absence of steroid injection. LH surges always occurred at lights out on a 14-h light, 10-h dark cycle on the day after EB injection but occurred on the same day as progesterone injection. The amount of EB or progesterone injected seemed unimportant but, in either case, had to be given within a limited diurnal period of sensitivity. LH surges comparable to those of intact proestrous females were produced either by injecting both EB and progesterone or by manipulating the background dose of encapsulated estradiol. In the latter regard, when ovariectomized females were implanted with a wide range of doses of estradiol (0.1--1000 microgram/capsule), a decided window phenomenon became apparent. That is, LH surging could be induced by steroid injections only within a limited range of background doses of encapsulated estradiol. The relationship of the above findings to the pheromonal control of LH secretion and ovulation in mice is as yet unclear.
在小鼠中研究了类固醇对促黄体生成素（LH）排卵前释放的调节作用，在该物种中，排卵受到启动信息素的强烈影响。对卵巢切除的小鼠，在硅胶胶囊中植入雌二醇以引入负反馈。然后通过注射苯甲酸雌二醇（EB）或孕酮诱导出类似排卵前的LH峰。在不注射类固醇的情况下未观察到LH峰。LH峰总是在注射EB后的第二天，在14小时光照、10小时黑暗周期的熄灯时出现，但在注射孕酮的同一天出现。注射的EB或孕酮的量似乎并不重要，但在任何一种情况下，都必须在有限的昼夜敏感期间内给予。通过同时注射EB和孕酮或通过操纵封装雌二醇的背景剂量，可产生与完整动情前期雌性相当的LH峰。在这方面，当给卵巢切除的雌性植入各种剂量的雌二醇（0.1 - 1000微克/胶囊）时，明显出现了一个明确的窗口现象。也就是说，只有在封装雌二醇的背景剂量的有限范围内，类固醇注射才能诱导LH峰。上述发现与小鼠中LH分泌和排卵的信息素控制之间的关系尚不清楚。
