Logan W J
Muscle Nerve. 1978 May-Jun;1(3):206-12. doi: 10.1002/mus.880010305.
An established rat muscle cell culture, L-6, was employed for the evaluation of muscle amino acid transport. The initial influx of 16 radiolabeled amino acids into these cells was determined, and an overall uptake profile was constructed. L-6 cells accumulate amino acids progressively with time by saturable, temperature-dependent processes. As found in other preparations, several amino acids have sodium-dependent uptake systems. There is a wide range in the rate of transport of the different amino acids. The overall profile of uptake is fairly reproducible and may be characteristic of muscle tissue; it does not resemble that previously found in other cell types. The profile of amino acid uptake into these cells compares favorably with the pattern of amino acid transfer from the bloodstream to muscle found in intact rats. It is concluded that muscle cell transport is basically similar to that of other cell types. L-6 muscle cells have an amino acid uptake profile which resembles that of intact muscle; they should thus prove useful in the further delineation of muscle amino acid transport mechanisms.
采用已建立的大鼠肌肉细胞培养物L-6来评估肌肉氨基酸转运。测定了16种放射性标记氨基酸进入这些细胞的初始流入量，并构建了总体摄取图谱。L-6细胞通过可饱和的、温度依赖性过程随时间逐渐积累氨基酸。正如在其他制剂中所发现的，几种氨基酸具有钠依赖性摄取系统。不同氨基酸的转运速率有很大差异。摄取的总体图谱相当可重复，可能是肌肉组织的特征；它与先前在其他细胞类型中发现的图谱不同。这些细胞中氨基酸摄取的图谱与完整大鼠中从血液到肌肉的氨基酸转运模式相比具有优势。得出的结论是，肌肉细胞转运与其他细胞类型基本相似。L-6肌肉细胞具有类似于完整肌肉的氨基酸摄取图谱；因此，它们在进一步阐明肌肉氨基酸转运机制方面应该会证明是有用的。
