水溶液中细胞色素c中缓慢交换肽质子的核磁共振研究。
Nuclear magnetic resonance studies of slowly exchanging peptide protons in cytochrome c in aqueous solution.
作者信息
Patel D J, Canuel L L
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1398-402. doi: 10.1073/pnas.73.5.1398.
Abstract
The slowly exchanging protons in oxidized and reduced horse heart cytochrome c (D20, uncorrected pH meter reading 6.5 room temperature) have been monitored by recording the 270 and 360 MHz proton nuclear magnetic resonance spectra of the reduced protein between 5 and 11 parts per million downfield from 2,2-dimethyl-2-silapentane-5-sulfonate.
摘要
通过记录还原态蛋白质在相对于2,2 - 二甲基 - 2 - 硅戊烷 - 5 - 磺酸盐的百万分之5至11的低场处270和360兆赫的质子核磁共振谱，监测了氧化态和还原态马心细胞色素c（重水，未校正pH计读数6.5，室温）中缓慢交换的质子。
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