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水溶液中细胞色素c中缓慢交换肽质子的核磁共振研究。

Nuclear magnetic resonance studies of slowly exchanging peptide protons in cytochrome c in aqueous solution.

作者信息

Patel D J, Canuel L L

出版信息

Proc Natl Acad Sci U S A. 1976 May;73(5):1398-402. doi: 10.1073/pnas.73.5.1398.

DOI:10.1073/pnas.73.5.1398
PMID:5721
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430301/
Abstract

The slowly exchanging protons in oxidized and reduced horse heart cytochrome c (D20, uncorrected pH meter reading 6.5 room temperature) have been monitored by recording the 270 and 360 MHz proton nuclear magnetic resonance spectra of the reduced protein between 5 and 11 parts per million downfield from 2,2-dimethyl-2-silapentane-5-sulfonate.

摘要

通过记录还原态蛋白质在相对于2,2 - 二甲基 - 2 - 硅戊烷 - 5 - 磺酸盐的百万分之5至11的低场处270和360兆赫的质子核磁共振谱，监测了氧化态和还原态马心细胞色素c（重水，未校正pH计读数6.5，室温）中缓慢交换的质子。

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本文引用的文献

1
Hydrogen-deuterium exchange of cytochrome c. I. Effect of oxidation state.细胞色素c的氢-氘交换。I.氧化态的影响。
Biochemistry. 1968 Aug;7(8):2710-7. doi: 10.1021/bi00848a003.
2
Hydrogen-deuterium exchange of cytochrome c. II. Effect of pH.细胞色素c的氢-氘交换。II. pH值的影响。
Biochemistry. 1968 Aug;7(8):2718-23. doi: 10.1021/bi00848a004.
3
Ferricytochrome c. I. General features of the horse and bonito proteins at 2.8 A resolution.高铁细胞色素c。I. 马和鲣鸟蛋白质在2.8埃分辨率下的一般特征。
J Biol Chem. 1971 Mar 10;246(5):1511-35.
4
Nuclear magnetic resonance study of cyanoferrimyoglobin; identification of pseudocontact shifts.氰化高铁肌红蛋白的核磁共振研究；赝接触位移的鉴定
J Mol Biol. 1970 Oct 14;53(1):35-48. doi: 10.1016/0022-2836(70)90044-6.
5
Cytochrome c.细胞色素c
Adv Protein Chem. 1966;21:113-286. doi: 10.1016/s0065-3233(08)60128-x.
6
A nuclear magnetic resonance study of bovine pancreatic trypsin inhibitor. Tyrosine titrations and backbone NH groups.牛胰蛋白酶抑制剂的核磁共振研究。酪氨酸滴定和主链NH基团。
Biochemistry. 1973 Mar 27;12(7):1323-9. doi: 10.1021/bi00731a012.
7
Proton magnetic resonance investigation of the conformational properties of the basic pancreatic trypsin inhibitor.胰腺碱性胰蛋白酶抑制剂构象性质的质子磁共振研究
FEBS Lett. 1973 Apr 1;31(1):114-8. doi: 10.1016/0014-5793(73)80086-9.
8
The structure and history of an ancient protein.一种古老蛋白质的结构与历史
Sci Am. 1972 Apr;226(4):58-70 passim. doi: 10.1038/scientificamerican0472-58.
9
Hydrogen exchange.氢交换
Annu Rev Biochem. 1972;41:903-24. doi: 10.1146/annurev.bi.41.070172.004351.
10
Pancreatic trypsin inhibitor (Kunitz). I. Structure and function.胰腺胰蛋白酶抑制剂（库尼茨）。I. 结构与功能。
Cold Spring Harb Symp Quant Biol. 1972;36:141-8. doi: 10.1101/sqb.1972.036.01.019.