Erices J
Arch Exp Veterinarmed. 1979 Jan;33(1):129-40.
Puberty was induced in 39 clinically prepuberal gilts (two groups of three sub-groups each) by parallel but locally separated application of 500 IU PMSG ("Maretropin") and 250 IU HCG ("Gonadex"), with the view to testing ways to synchronise ovulation. Seventy-two hours were allowed to elapse, before 24 animals received another application of 500 IU HCG and 15 animals 250 IU HCG. The animals were slaughtered in consecutive groups of study ovulation and histolotically examined to disclose endometrial processes. Ovulations were found to be well synchronised in the recipients of a second injection of 500 IU HCG. Only sub-threshold effects with no synchronised ovulation were recorded from the animals that had received a second dose of 250 IU HCG. A second injection of 500 IU HCG should be given not until something between 78 and 82 hours after puberty induction for optimum follicle maturation and adequate proliferation of the endometrium.
为了测试同步排卵的方法,对39头临床未进入青春期的小母猪(分为两组,每组三个亚组)进行青春期诱导,通过平行但局部隔开的方式分别注射500国际单位的孕马血清促性腺激素(“Maretropin”)和250国际单位的人绒毛膜促性腺激素(“Gonadex”)。72小时后,24头母猪再次注射500国际单位的人绒毛膜促性腺激素,15头母猪注射250国际单位的人绒毛膜促性腺激素。将这些母猪按连续批次宰杀以研究排卵情况,并进行组织学检查以揭示子宫内膜的变化过程。结果发现,第二次注射500国际单位人绒毛膜促性腺激素的母猪排卵同步良好。而接受第二次注射250国际单位人绒毛膜促性腺激素的母猪仅记录到阈下效应,未出现同步排卵。为了实现最佳的卵泡成熟和子宫内膜的充分增殖,应在青春期诱导后78至82小时之间才进行第二次500国际单位人绒毛膜促性腺激素的注射。