玉米芽细胞内细胞器的甾醇和甾醇酯研究。

Studies on the sterols and sterol esters of the intracellular organelles of maize shoots.

Kemp R J, Mercer E I

Biochem J. 1968 Nov;110(1):119-25. doi: 10.1042/bj1100119.

Abstract

The composition of the esterified and unesterified sterols of the nuclear, chloroplastidic, mitochondrial and microsomal fractions of 21-day-old maize shoots was examined. 2. The microsomal and mitochondrial fractions contain the bulk of the sterols of the tissue. 3. Only 1% of the sterol isolated from all the organelles is esterified. 4. The nuclear fraction has the greatest proportion of esterified sterol and the microsomal fraction the least. 5. 4-Demethyl sterols constitute the bulk of both esterified and unesterified sterols in all organelle fractions. 6. Cholesterol is the major esterified 4-demethyl sterol of the nuclear and chloroplastidic fractions, but only the nuclear fraction has an appreciable proportion of unesterified cholesterol. 7. Sterol esters of linolenic acid are more abundant in the mitochondrial and microsomal fractions than in the other two fractions.

摘要

对21日龄玉米芽的细胞核、叶绿体、线粒体和微粒体部分的酯化和未酯化甾醇组成进行了检测。2. 微粒体和线粒体部分含有组织中大部分的甾醇。3. 从所有细胞器中分离出的甾醇只有1%是酯化的。4. 细胞核部分酯化甾醇的比例最高，微粒体部分最低。5. 4-去甲基甾醇在所有细胞器部分的酯化和未酯化甾醇中均占大部分。6. 胆固醇是细胞核和叶绿体部分主要的酯化4-去甲基甾醇，但只有细胞核部分有相当比例的未酯化胆固醇。7. 线粒体和微粒体部分中亚麻酸甾醇酯比其他两个部分更为丰富。