核糖核酸酶H、核糖核酸内切酶和磷酸酶的测定。
An assay of ribonuclease H, endoribonucleases, and phosphatases.
作者信息
Starvianopoulos J G, Chargaff E
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1556-8. doi: 10.1073/pnas.73.5.1556.
Abstract
An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely. The method is applicable to other endoribonucleases, phosphodiesterases, and phosphatases.
摘要
本文描述了一种核糖核酸酶H（EC 3.1.4.34）的测定方法，该方法可用于估算该酶作用于DNA-RNA杂交底物的核糖部分时产生的切割数量和类型。在测试的六种不同的均聚物杂交体中，酶诱导的断裂数量差异很大。该方法适用于其他核糖核酸内切酶、磷酸二酯酶和磷酸酶。
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