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[食用蘑菇(田野蘑菇,施夫,前弗)中镉结合蛋白的测定(作者译)]

[Determination of cadmium binding proteins in the edible mushroom (Agaricus arvensis Schff, ex Fr.) (author's transl)].

作者信息

Kruse H, Lommel A

出版信息

Z Lebensm Unters Forsch. 1979 Jun 29;168(6):444-7. doi: 10.1007/BF01479258.

Abstract

The isolation and characterization of cadmium compounds from Agaricus arvensis Schff. ex Fr. are described. These compounds were extractable with buffers. Ultrafiltrations and protein precipitations suggested protein bound cadmium. The mushroom proteins were separated by gel chromatography and the cadmium content in the corresponding fractions was determined by flameless atomic absorption. Two cadmium containing protein fractions were found with cadmium concentrations of 2.1 and 1 mg/g protein, respectively. The molecular weights of both cadmium containing proteins were estimated to be about 2,000 and 15,000--20,000, respectively by comparison with standard proteins.

摘要

描述了从野蘑菇(Agaricus arvensis Schff. ex Fr.)中分离和鉴定镉化合物的过程。这些化合物可用缓冲液提取。超滤和蛋白质沉淀表明镉与蛋白质结合。通过凝胶色谱法分离蘑菇蛋白质,并通过无火焰原子吸收法测定相应组分中的镉含量。发现了两个含镉蛋白质组分,镉浓度分别为2.1和1毫克/克蛋白质。通过与标准蛋白质比较,两种含镉蛋白质的分子量估计分别约为2000和15000 - 20000。

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