一种新型抗肿瘤抗生素——孢子霉素的作用方式

The mode of action of a new antitumor antibiotic, sporamycin.

作者信息

Okamoto M, Komiyama K, Takeshima H, Yamamoto H, Umezawa I

出版信息

J Antibiot (Tokyo). 1979 Apr;32(4):386-91. doi: 10.7164/antibiotics.32.386.

DOI:10.7164/antibiotics.32.386

Abstract

Sporamycin, an antitumor antibiotic, primarily inhibited DNA synthesis, while RNA and protein synthesis were not significantly affected in HeLa S3 cells. The antibiotic also caused strand scission of cellular DNA. However, the effects were not observed when the cells were incubated at 0 degrees C before washing and subsequently incubated at 37 degrees C. The Tm of calf thymus DNA decreased when incubated with sporamycin in vitro. Sporamycin did not affect DNA synthesis in vitro catalyzed by partially purified DNA polymerase alpha, beta, and gamma derived from EHRLICH ascites cells.

摘要

孢子霉素是一种抗肿瘤抗生素，主要抑制DNA合成，而对HeLa S3细胞中的RNA和蛋白质合成没有显著影响。该抗生素还会导致细胞DNA链断裂。然而，在洗涤前将细胞于0℃孵育，随后于37℃孵育时，未观察到这些效应。体外与孢子霉素一起孵育时，小牛胸腺DNA的熔点降低。孢子霉素不影响由源自艾氏腹水瘤细胞的部分纯化的DNA聚合酶α、β和γ催化的体外DNA合成。