Martensz N D, Scaramuzzi R J
J Endocrinol. 1979 Jun;81(3):249-59. doi: 10.1677/joe.0.0810249.
Levels of plasma LH, FSH and progesterone during the breeding season were measured by radioimmunoassay in control ewes and ewes actively immunized against androstenedione-11 alpha-hemisuccinyl--bovine serum albumin or testosterone-3(O-carboxymethyl)oxime--bovine serum albumin. Immunization against androstenedione resulted in normal oestrous cycles with raised plasma LH and progesterone levels and a reduction in the concentration of FSH during the luteal phase. It is tentatively suggested that androstenedione, or its metabolites, could modify the oestrogenic control of LH secretion and facilitate the release of FSH in the ewe. Immunization against testosterone prevented oestrus and resulted in markedly increased levels of LH without alteration of the FSH concentration. Since evidence of increased binding of oestradiol-17 beta was found in the ewes immunized against testosterone, these results cannot be attributed solely to a reduction in the biologically active faction of testosterone.
在繁殖季节，通过放射免疫分析法测定了对照母羊以及主动免疫雄烯二酮-11α-半琥珀酰-牛血清白蛋白或睾酮-3（O-羧甲基）肟-牛血清白蛋白的母羊血浆中促黄体生成素（LH）、促卵泡生成素（FSH）和孕酮的水平。针对雄烯二酮的免疫导致正常的发情周期，黄体期血浆LH和孕酮水平升高，FSH浓度降低。初步认为，雄烯二酮或其代谢产物可能改变雌激素对LH分泌的控制，并促进母羊体内FSH的释放。针对睾酮的免疫阻止了发情，并导致LH水平显著升高，而FSH浓度未改变。由于在免疫睾酮的母羊中发现了雌二醇-17β结合增加的证据，这些结果不能仅仅归因于睾酮生物活性部分的降低。
