Barendsz A W
Int J Environ Anal Chem. 1979;6(2):89-94. doi: 10.1080/03067317908071163.
The enzyme butyrylcholinesterase from horse serum catalyses the hydrolysis of certain esters. The orange-red 2,6-dichloroindophenyl acetate will be converted by the enzyme into a deep blue alcohol. The colour transformation does not occur when the enzyme is inactivated. By making use of this biochemical reaction a cheap and simple, but very sensitive and specific detection tube could bedeveloped. The tube comprises a breakable ampoule with an aqueous buffer solution, a freeze-dried preparation of the chromogenic ester with a filler promoting its dissolution, a freeze-dried preparation of butyrylcholinesterase with a filler promoting its stability, and an indication layer. DDVP can be detected at concentrations as low as 0.4 mg/m3, when the sampled airvolume is 21.
马血清中的丁酰胆碱酯酶可催化某些酯类的水解。橙红色的2,6 - 二氯吲哚苯基乙酸酯会被该酶转化为深蓝色的醇。当酶失活时，颜色不会发生转变。利用这一生物化学反应，可以开发出一种廉价、简单但非常灵敏且特异的检测管。该检测管包括一个装有水性缓冲溶液的易碎安瓿、带有促进其溶解的填充剂的显色酯冻干制剂、带有促进其稳定性的填充剂的丁酰胆碱酯酶冻干制剂以及一个指示层。当采样空气体积为21时，敌敌畏的检测浓度低至0.4毫克/立方米。
