Kosakai M, Yosizawa Z
J Biochem. 1979 Jul;86(1):147-53.
The stability of ester sulfates in porcine intestinal heparin to solvolytic desulfation (100 degrees C, 9 H) with dimethylsulfoxide containing 2% pyridine was examined, in comparison with the case of dilute acid treatment (0.1 M HCl, 100 degrees C, 70 min). The resulting heparin modifications were deaminated and the deamination products were fractionated by the procedures reported previously (1978) J. Biochem. 83, 1567-1575). The yields of disulfated disaccharide (b-2) and monosulfated disaccharides (e-2-1 and e-2-3) indicated that 2-O-sulfates in L-iduronic acid residues of heparin were more libile than 6-O-sulfates in glucosamine residues to the dilute acid treatment, whereas the opposite was the case for the solvolysis. The product of heparin modification by solvolysis was similar to whale heparin in the distribution of ester sulfates.
研究了猪肠肝素中的硫酸酯在含2%吡啶的二甲基亚砜中进行溶剂解脱硫（100℃，9小时）时的稳定性，并与稀酸处理（0.1M HCl，100℃，70分钟）的情况进行了比较。对所得的肝素修饰物进行脱氨处理，并按照先前报道的方法（1978年，《生物化学杂志》83卷，1567 - 1575页）对脱氨产物进行分级分离。二硫酸化二糖（b - 2）和单硫酸化二糖（e - 2 - 1和e - 2 - 3）的产率表明，肝素中艾杜糖醛酸残基上的2 - O - 硫酸酯在稀酸处理下比氨基葡萄糖残基上的6 - O - 硫酸酯更不稳定，而在溶剂解时情况则相反。通过溶剂解得到的肝素修饰产物在硫酸酯分布上与鲸肝素相似。
