双插入染料在DNA组成“原位”研究中的应用。
Employment of bis-intercalating dyes for the "in situ" study of DNA composition.
作者信息
Bottiroli G, Giordano P, Doglia S, Cionini P G
出版信息
Basic Appl Histochem. 1979;23(1):59-63.
PMID:574384
Abstract
Spectrofluorometric characteristics of DNA-RO4 complexes have been studied in order to evaluate the possibility of employment of RO4 (a bis-intercalating dye) for the determination of the DNA base composition. The results have shown the usefulness of this compound for DNA with AT percentage between 30% and 60%. RO4 has been also applied to the study of the chromosome banding mechanism.
摘要
为了评估双嵌入染料RO4用于测定DNA碱基组成的可能性，研究了DNA-RO4复合物的荧光光谱特性。结果表明，该化合物对AT含量在30%至60%之间的DNA有用。RO4也已应用于染色体显带机制的研究。
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