Mears G J, Van Petten G R, Harris W H, Bell J U, Lorscheider F L
J Reprod Fertil. 1979 Nov;57(2):461-7. doi: 10.1530/jrf.0.0570461.
A programme consisting of 14 daily injections of progesterone (10 mg) followed by single injection of PMSG (500 i.u.) and oestradiol- 17 beta (30 micrograms), along with controlled temperature (18-20 degrees C) and lighting (10 h light/24 h), was applied to 60 anoestrous ewes between late May and early August to induce reproductive activity. Breeding started within 24 h of the oestradiol injection and 80.0% of the ewes conceived at the induced oestrus. Dorset ewes had higher conception (95.2 versus 71.8%) and prolificacy (1.74 versus 1.52 fetuses/ewe) rates than did crossbred Suffolk ewes. Plasma progesterone concentrations during progesterone administration were significantly higher than those found during anoestrus and were generally lower in ewes which did not conceive than in those which did. The plasma progesterone data indicated that ovulation had occurred in most of the ewes which were not pregnant at 90-100 days and that many may have been pregnant initially but then lost the conceptus.
在5月下旬至8月初期间,对60只处于乏情期的母羊实施了一项方案,该方案包括每天注射14次孕酮(10毫克),随后单次注射孕马血清促性腺激素(500国际单位)和雌二醇-17β(30微克),同时控制温度(18 - 20摄氏度)和光照(10小时光照/24小时),以诱导生殖活动。在注射雌二醇后24小时内开始配种,80.0%的母羊在诱导发情期受孕。多塞特母羊的受孕率(95.2%对71.8%)和产仔率(1.74只胎儿/母羊对1.52只胎儿/母羊)高于杂交萨福克母羊。在注射孕酮期间,血浆孕酮浓度显著高于乏情期时的浓度,并且未受孕母羊的血浆孕酮浓度通常低于受孕母羊。血浆孕酮数据表明,在90 - 100天时未怀孕的大多数母羊已经排卵,而且许多母羊可能最初已怀孕,但随后失去了胚胎。