Johnson B C, Preston J F
Arch Microbiol. 1979 Aug 6;122(2):161-7. doi: 10.1007/BF00411355.
In order to understand the basis upon which amanitin-accumulating species of Amanita are able to develop in the presence of these specific inhibitors of RNA synthesis, the in vitro RNA synthesizing activities of nuclei isolated from amanitin-accumulating species. Amanita hygroscopica (culture, derived from amanitin-accumulating carpophore) and A. suballiacea (carpophore), and from the non-accumulating species A. solitaria (culture) and A. brunnescens (carpophore) were tested for their sensitivities to alpha-amanitin inhibition. The nuclear RNA synthesizing activities obtained from both carpophores and cultures of non-accumulating organisms displayed significant sensitivities to alpha-amanitin, whereas those obtained from accumulating organisms displayed remarkable resistance to alpha-amanitin. The observed relationship between levels of amanitins in carpophores and resistance of the RNA synthesizing activities to inhibition by alpha-amanitin supports the hypothesis that amanitins may function as regulators of mRNA transcription in Amanita species.
为了理解鹅膏菌中积累鹅膏毒素的物种在这些RNA合成特异性抑制剂存在的情况下能够生长的基础，对从积累鹅膏毒素的物种、小托柄鹅膏（培养物，源自积累鹅膏毒素的子实体）和近蒜味鹅膏（子实体），以及非积累物种独居鹅膏（培养物）和浅褐鹅膏（子实体）中分离出的细胞核的体外RNA合成活性进行了α-鹅膏毒素抑制敏感性测试。从非积累生物的子实体和培养物中获得的细胞核RNA合成活性对α-鹅膏毒素表现出显著敏感性，而从积累生物中获得的细胞核RNA合成活性对α-鹅膏毒素表现出显著抗性。在子实体中鹅膏毒素水平与RNA合成活性对α-鹅膏毒素抑制的抗性之间观察到的关系支持了这样的假设，即鹅膏毒素可能在鹅膏菌物种中作为mRNA转录的调节因子发挥作用。
