Zhang Ping, Chen Zuohong, Hu Jinsong, Wei Baoyang, Zhang Zhiguang, Hu Wenqi
College of Life Science, Hunan Normal University, Changsha, PR China.
FEMS Microbiol Lett. 2005 Nov 15;252(2):223-8. doi: 10.1016/j.femsle.2005.08.049. Epub 2005 Sep 15.
Amanita exitialis Zhu L. Yang and T.H. Li is a lethal mushroom species recently isolated in Guangdong Province, China. In this report, a pure culture of this species was obtained for the first time. To confirm the identity of the pure culture, the internal transcribed spacer regions of the nuclear ribosomal DNA of the pure culture and of a typical fruiting body of the species were sequenced and compared. Further, amatoxins produced by pure cultures were analyzed and characterized by high-performance liquid chromatography and mass spectrometry analysis. The results showed that the pure cultures produced 728.3 +/- 43.8 microg g(-1) (dry matter) of alpha-Amanitin and 60.0 +/- 20.7 microg g(-1) (dry matter) of beta-Amanitin, respectively, a yield which is about 10% of that produced by fruiting bodies.
致命鹅膏是中国广东省最近分离出的一种剧毒蘑菇物种。在本报告中，首次获得了该物种的纯培养物。为了确认纯培养物的身份，对纯培养物和该物种典型子实体的核糖体DNA内部转录间隔区进行了测序和比较。此外，通过高效液相色谱和质谱分析对纯培养物产生的鹅膏毒素进行了分析和表征。结果表明，纯培养物分别产生了728.3±43.8微克/克（干物质）的α-鹅膏菌素和60.0±20.7微克/克（干物质）的β-鹅膏菌素，产量约为子实体产量的10%。
