Drozhevkina M S
J Hyg Epidemiol Microbiol Immunol. 1979;23(3):340-7.
The authors formed a collection of typing phages in order to differentiate cholera vibrios of both biotypes. The collection consists of 7 phages proposed by Mukerjee for typing vibrios of the classical biotype and 3 El Tor phages isolated at the period of the 7th pandemy of cholera. In forming the typing collection the authors observed the following principles: 1) to crate a single set of typing phages and a single scheme of phage type differentiation of classical cholera and El Tor vibrios; 2) to use virulent phages; 3) to form a collection of serologically different phage types. The proposed collection allows to reveal a larger number of phage types as compared with phages proposed by Mukerjee phage typing a larger number of strains enhancing thus the epidemiological significance of the method of phage typing cholera vibrios.
作者组建了一组分型噬菌体,以区分两种生物型的霍乱弧菌。该噬菌体组由穆克吉提出的用于对经典生物型弧菌进行分型的7种噬菌体和在霍乱第七次大流行期间分离出的3种埃尔托噬菌体组成。在组建分型噬菌体组时,作者遵循了以下原则:1)创建一套单一的分型噬菌体以及经典霍乱弧菌和埃尔托弧菌噬菌体分型区分的单一方案;2)使用烈性噬菌体;3)形成一组血清学不同的噬菌体类型。与穆克吉提出的噬菌体相比,所提议的噬菌体组能够揭示更多的噬菌体类型,可对更多菌株进行噬菌体分型,从而提高了霍乱弧菌噬菌体分型方法的流行病学意义。