Gras G, Hasselmann C, Pellissier C, Mondain J
Toxicol Eur Res. 1979 Jul;2(4):199-206.
The methiocarb (4-methylthio 3,5-dimethylphenyl N-methylcarbamate) has repellent properties towards against Quelea, a small bird deteriorating crops in Africa. The residue analysis of the carbamate compound and of its possible metabolites on treated Sorghum sp. was completed by TLC after chloroformic quantitative extraction. The isolation of metabolites by two dimensional thin layer chromatography demonstrates the presence of methiocarb sulfone. The quantitative survey of residues was achieved by the treatment of the methiocarb by hydrolyzed derivatives by dansylchloride, a very fluorescent component. The fluorescent densitometry of dansylated by methylamine allows a total measurement of the residues at a level of less than 10 ng. This investigation reveals the disappearance curve of methiocarb in our samples in process of time and to define the half-live and, therefore, the intervals of treatment. Taking into account the RI 50 index, our results provide encouraging support for the utilization of methiocarb in the eradication of the harmful Quelea.
灭虫威（4-甲硫基-3,5-二甲基苯基N-甲基氨基甲酸酯）对非洲一种破坏农作物的小鸟——红嘴奎利亚雀具有驱避特性。在经氯仿定量萃取后，通过薄层色谱法完成了该氨基甲酸酯化合物及其可能代谢物在处理过的高粱属植物上的残留分析。通过二维薄层色谱法分离代谢物，证明了灭虫威砜的存在。通过用丹磺酰氯（一种荧光很强的成分）处理灭虫威的水解衍生物来进行残留量的定量检测。用甲胺对丹磺酰化产物进行荧光光密度测定，可对低于10纳克水平的残留量进行全面测量。这项研究揭示了我们样本中灭虫威随时间的消失曲线，并确定了半衰期，从而确定了处理间隔。考虑到RI 50指数，我们的结果为使用灭虫威消灭有害的红嘴奎利亚雀提供了令人鼓舞的支持。
