尿素八叠球菌与芽孢杆菌属成员之间的亲缘关系。
Genetic relationship between Sarcina ureae and members of the genus Bacillus.
作者信息
Herndon S E, Bott K F
出版信息
J Bacteriol. 1969 Jan;97(1):6-12. doi: 10.1128/jb.97.1.6-12.1969.
Deoxyribonucleic acid-ribonucleic acid (DNA-RNA) and DNA-DNA hybridization studies were performed to determine the degree of genetic relatedness between Sarcina ureae and several members of the genus Bacillus. DNA-RNA hybridization showed a high degree of homology between S. ureae RNA and DNA from Bacillus species having a similar guanine plus cytosine content. The DNA from other genera of the family Micrococcaceae showed less homology with S. ureae RNA than did that of the Bacillus species tested; however, this homology was not found between the DNA of S. ureae and DNA from these Bacillus species or DNA from the other Micrococcaceae tested. Transformation with Bacillus DNA, infection with representatives from several major classes of Bacillus phages, and electrophoretic analysis of proteins in crude extracts of these strains were also attempted as a further test of the genetic relationship between the genera. These experiments did not support the belief that the two groups are closely related genetically.
进行了脱氧核糖核酸-核糖核酸（DNA-RNA）和DNA-DNA杂交研究，以确定脲八叠球菌与芽孢杆菌属的几个成员之间的遗传相关性程度。DNA-RNA杂交显示，脲八叠球菌RNA与来自鸟嘌呤加胞嘧啶含量相似的芽孢杆菌属物种的DNA具有高度同源性。微球菌科其他属的DNA与脲八叠球菌RNA的同源性低于所测试的芽孢杆菌属物种；然而，在脲八叠球菌的DNA与这些芽孢杆菌属物种的DNA或所测试的其他微球菌科的DNA之间未发现这种同源性。还尝试用芽孢杆菌DNA进行转化、用几种主要类别的芽孢杆菌噬菌体的代表进行感染以及对这些菌株粗提取物中的蛋白质进行电泳分析，作为对这两个属之间遗传关系的进一步测试。这些实验不支持这两组在遗传上密切相关的观点。
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