Braude R, Altman R, Rouvier J, Aguilar N
Thromb Haemost. 1977 Feb 28;37(1):136-43.
In vitro degradation of fibrinogen results in the appearance of an early fragment, different in its electrophoretic mobility to that of fragments X, Y, D and E. It reacts with antifibrinogen antiserum does not react with anti-D antiserum and it is antigenically related to fragment E. Compared with terminal fragment E, it migrates more slowly towards the anode, it appears earlier and in a greater concentration at the beginning, decreasing and disappearing during degradation. The antigen antibody crossed electrophoresis was used in these experiments, but to distinguish the fragments from already known X, Y or even fibrinogen, the applying of an intermediate gel with anti D was of great value.
纤维蛋白原的体外降解会产生一个早期片段，其电泳迁移率与片段X、Y、D和E不同。它与抗纤维蛋白原抗血清反应，不与抗D抗血清反应，且在抗原性上与片段E相关。与末端片段E相比，它向阳极迁移得更慢，在降解开始时出现得更早且浓度更高，在降解过程中浓度降低并消失。这些实验中使用了抗原抗体交叉电泳，但为了将这些片段与已知的X、Y甚至纤维蛋白原区分开来，使用含抗D的中间凝胶非常有价值。
