Immunoelectrophoretic studies on the kinetics of plasmic degradation of human fibrinogen.

In vitro degradation of fibrinogen results in the appearance of an early fragment, different in its electrophoretic mobility to that of fragments X, Y, D and E. It reacts with antifibrinogen antiserum does not react with anti-D antiserum and it is antigenically related to fragment E. Compared with terminal fragment E, it migrates more slowly towards the anode, it appears earlier and in a greater concentration at the beginning, decreasing and disappearing during degradation. The antigen antibody crossed electrophoresis was used in these experiments, but to distinguish the fragments from already known X, Y or even fibrinogen, the applying of an intermediate gel with anti D was of great value.