Sartor P
Acta Endocrinol (Copenh). 1977 Apr;84(4):804-12. doi: 10.1530/acta.0.0840804.
The uterine uptake of tritiated hormones, oestradiol and progesterone, was studied at the time of ovum implantation in the rat. Macromolecular dyes such as trypan blue were used to distinguish implanted sites from the unimplanted ones. A differential uptake was found between the implanted and unimplanted sites in normal 6th day pregnant females. Oestradiol was retained more at the unimplanted sites, whereas its half-life was approximately the same at the two levels. On the other hand progesterone was found in greater amounts in the implanted sites due to a difference in half-life for each site (30 min for unimplanted sites, 40 min for implanted ones during the first 30 min following injection). When endogenous oestradiol retention was suppressed by ovariectomy, higher uptake of [3H] oestradiol in unimplanted sites was not found. These results suggest a heterogeneity in the binding sites of oestradiol in the pregnant uterus.
在大鼠卵子植入时，研究了子宫对氚标记激素雌二醇和孕酮的摄取情况。使用诸如台盼蓝等大分子染料来区分植入部位和未植入部位。在正常怀孕第6天的雌性大鼠中，发现植入部位和未植入部位之间存在差异摄取。雌二醇在未植入部位保留更多，而其半衰期在两个水平大致相同。另一方面，由于每个部位半衰期不同（注射后最初30分钟内，未植入部位为30分钟，植入部位为40分钟），孕酮在植入部位含量更高。当通过卵巢切除术抑制内源性雌二醇保留时，未发现未植入部位对[3H]雌二醇的摄取增加。这些结果表明，怀孕子宫中雌二醇结合位点存在异质性。
