Welsch C W, Adams C, Lambrecht L K, Hassett C C, Brooks C L
Br J Cancer. 1977 Mar;35(3):322-8. doi: 10.1038/bjc.1977.46.
Chronic administration of 17beta-oestradiol (via drinking water) or the oral contraceptive Enovid (norethynodrel and mestranol) (0-1 mg injected s.c. twice weekly) to nulliparous C3H/HeJ female mice, beginning at one month of age and terminating at 20 months (17beta-oestradiol) or 22 months (Enovid), significantly increased the incidence of mammary tumours over solvent-treated controls. Concurrent treatment of the steroid-treated mice with 2-bromo-alpha-ergocryptine (CB-154) (0-1 mg s.c. injected daily) significantly reduced mammary tumour incidence and mammary hyperplastic nodule development to the control level. CB-154 is an efficacious inhibitor of pituitary prolactin secretion. These results demonstrate that steroid-induced mammary gland dysplasias can be sharply reduced by chronic CB-154 treatment, and suggest that some of the mammary tumorigenic activities of oestrogenic steroids in C3H mice are mediated via an increased secretion of pituitary prolactin.
从1月龄开始，对未生育的C3H/HeJ雌性小鼠持续经饮水给予17β-雌二醇，或皮下注射口服避孕药炔诺酮（norethynodrel）和炔雌醇甲醚（mestranol）的复方炔诺酮片（每周两次，每次0 - 1 mg），17β-雌二醇组至20月龄结束，复方炔诺酮片组至22月龄结束，与溶剂处理的对照组相比，显著增加了乳腺肿瘤的发生率。对接受类固醇处理的小鼠同时给予2-溴-α-麦角隐亭（CB - 154）（每天皮下注射0 - 1 mg），可使乳腺肿瘤发生率和乳腺增生性结节的发展显著降低至对照水平。CB - 154是垂体催乳素分泌的有效抑制剂。这些结果表明，长期给予CB - 154可显著减少类固醇诱导的乳腺发育异常，提示雌激素类固醇在C3H小鼠中的一些致乳腺肿瘤活性是通过垂体催乳素分泌增加介导的。
