Onley J H
J Assoc Off Anal Chem. 1977 May;60(3):679-81.
A gas-liquid chromatographic (GLC) method has been developed for the determination of captan (N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide) and 2 metabolites, tetra-hydrophthalimide (THPI) and tetrahydrophthalamic acid (THPMA), in milk and meat. The sample is extracted with ethyl acetate and is cleaned up by acetonitrile partition and silica gel chromatography where captan, THPI, and THPMA are separated. Captan is directly determined by GLC. THPI and THPMA are separately derivatized in an acetone solution of pentafluorobenzyl bromide. The resultant derivatives are purified separately on an Al2O3 column and quantitated by GLC, using an electron capture detector. Recoveries from milk samples fortified at 0.02-10 ppm ranged from 71 to 102%; recoveries from meat samples fortified at 0.04-10 ppm ranged from 75 to 99%.
已开发出一种气液色谱（GLC）法，用于测定牛奶和肉类中的克菌丹（N-三氯甲硫基-4-环己烯-1,2-二甲酰亚胺）及其两种代谢物，四氢邻苯二甲酰亚胺（THPI）和四氢邻苯二甲酸（THPMA）。样品用乙酸乙酯萃取，通过乙腈分配和硅胶色谱法净化，在此过程中克菌丹、THPI和THPMA得以分离。克菌丹直接通过GLC测定。THPI和THPMA分别在五氟苄基溴的丙酮溶液中衍生化。所得衍生物分别在氧化铝柱上纯化，并使用电子捕获检测器通过GLC进行定量。在0.02 - 10 ppm加标水平下，牛奶样品的回收率在71%至102%之间；在0.04 - 10 ppm加标水平下，肉类样品的回收率在75%至99%之间。
