Reichenbach H, Voelz H, Dworkin M
J Bacteriol. 1969 Feb;97(2):905-11. doi: 10.1128/jb.97.2.905-911.1969.
Suspension cultures of Stigmatella aurantiaca (Chondromyces aurantiacus) were induced to form myxospores by addition of glycerol to the growing culture. The cells were fixed at various stages during conversion, thin sections were prepared, and changes in fine structure were studied. Vegetative cells are quite similar in their ultrastructure to Myxococcus xanthus. During transformation into myxospores, three important cytological changes were observed. Granules of storage material, probably polysaccharide and polyphosphate, accumulated; a 200 to 300-mum thick capsule was laid down, and the outer triple layer of the cell wall became locally folded. These cell wall folds were often densely packed and lay in pockets formed by the cytoplasmic membrane. We have suggested the possibility that the cell may store in these folds wall material which has become superfluous by the decrease in surface area during conversion.
通过向生长的橙色粘球菌（软骨霉菌）悬浮培养物中添加甘油，诱导其形成粘孢子。在转化过程中的不同阶段对细胞进行固定，制备超薄切片，并研究其精细结构的变化。营养细胞的超微结构与黄色粘球菌非常相似。在转化为粘孢子的过程中，观察到三个重要的细胞学变化。储存物质颗粒，可能是多糖和多磷酸盐，积累起来；形成了一层200至300微米厚的荚膜，细胞壁的外层三层局部折叠。这些细胞壁褶皱通常紧密堆积，位于由细胞质膜形成的袋囊中。我们提出了一种可能性，即细胞可能在这些褶皱中储存由于转化过程中表面积减小而多余的细胞壁物质。
