大鼠体内十二烷基[35-S]硫酸盐的代谢

The metabolism of potassium dodecyl [35-S]sulphate in the rat.

作者信息

Denner W H, Olavesen A H, Powell G M, Dodgson K S

出版信息

Biochem J. 1969 Jan;111(1):43-51. doi: 10.1042/bj1110043.

DOI:10.1042/bj1110043

PMID:5773748

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187492/

Abstract

The metabolic fate of potassium dodecyl [(35)S]sulphate was studied in rats. Intraperitoneal and oral administration of the ester into free-ranging animals were followed by the excretion of the bulk of the radioactivity in the urine within 12hr., approximately 17% being eliminated as inorganic [(35)S]sulphate. Similar results were obtained in experiments in which potassium dodecyl [(35)S]sulphate was injected intravenously into anaesthetized rats with bile-duct and ureter cannulae. Analysis of urinary radioactivity revealed the presence of a new ester sulphate (metabolite A). This metabolite was isolated, purified and subsequently identified as the sulphate ester of 4-hydroxybutyric acid by paper, thin-layer and gas chromatography, by paper electrophoresis and by comparison of its properties with those of authentic butyric acid 4-sulphate. The identity of the metabolite was confirmed by isotope-dilution experiments. When either purified metabolite A or authentic potassium butyric acid 4[(35)S]-sulphate was administered to free-ranging rats the bulk of the radioactivity was eliminated unchanged in the urine within 12hr., approx. 20% of the dose appearing as inorganic [(35)S]sulphate. Whole-body radioautography and isolated-liver-perfusion experiments implicated the liver as the major site of metabolism of potassium dodecyl [(35)S]sulphate. It is suggested that butyric acid 4-sulphate probably arises by omega-oxidation of dodecyl sulphate to a fatty acid-like compound, which is then degraded by beta-oxidation.

摘要

研究了大鼠体内十二烷基[（35）S]硫酸盐的代谢命运。将该酯经腹腔和口服给予自由活动的动物后，12小时内大部分放射性物质随尿液排出，约17%以无机[（35）S]硫酸盐形式被清除。在将十二烷基[（35）S]硫酸钾静脉注射到装有胆管和输尿管插管的麻醉大鼠的实验中也得到了类似结果。对尿液放射性的分析显示存在一种新的硫酸酯（代谢物A）。该代谢物被分离、纯化，随后通过纸色谱、薄层色谱和气相色谱、纸电泳，并通过将其性质与 authentic 4-硫酸丁酸的性质进行比较，鉴定为4-羟基丁酸的硫酸酯。通过同位素稀释实验证实了该代谢物的身份。当将纯化的代谢物A或 authentic 4-[(35)S]-硫酸丁酸钾给予自由活动的大鼠时，大部分放射性物质在12小时内未改变地随尿液排出，约20%的剂量以无机[（35）S]硫酸盐形式出现。全身放射自显影和离体肝脏灌注实验表明肝脏是十二烷基[（35）S]硫酸盐代谢的主要部位。有人提出，4-硫酸丁酸可能是由十二烷基硫酸盐经ω-氧化生成类似脂肪酸的化合物，然后再经β-氧化降解产生的。