Bayer M H
Plant Physiol. 1969 Feb;44(2):267-71. doi: 10.1104/pp.44.2.267.
Acidic indole auxins have been extracted from N. glauca, N. langsdorffii and their 2 tumor-prone 4n- and 2n-hybrids. After purification of the extracts and thin-layer chromatography, acidic indoles were subjected to esterification and gas chromatography. The esters of 4 indole acids were detected and determined: indole-3-acetic acid, indole-3-carboxylic acid, indole-3-propionic acid and indole-3-butyric acid. The indolic nature of fractionated samples was confirmed by spectrophotofluorometry and the physiological significance of the indole esters proven in a biotest. A substantial increase in extractable indole-3-butyric acid in the tumor-prone hybrids suggests an additional pathway of auxin synthesis in these tissues.
已从黄花烟草、郎氏烟草及其2种易发生肿瘤的4倍体和2倍体杂种中提取出酸性吲哚生长素。提取物经纯化和薄层色谱法处理后，对酸性吲哚进行酯化和气相色谱分析。检测并确定了4种吲哚酸的酯类：吲哚-3-乙酸、吲哚-3-羧酸、吲哚-3-丙酸和吲哚-3-丁酸。通过分光光度荧光法证实了分级样品的吲哚性质，并在生物试验中证明了吲哚酯的生理意义。易发生肿瘤的杂种中可提取的吲哚-3-丁酸大幅增加，表明这些组织中生长素合成存在额外途径。
