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含羞草上胚轴组织成熟过程中核糖核酸组分的变化

Changes in ribonucleic acid fractions during maturation of mimosa epicotyl tissues.

作者信息

Brown G N

出版信息

Plant Physiol. 1969 Feb;44(2):272-6. doi: 10.1104/pp.44.2.272.

DOI:10.1104/pp.44.2.272
PMID:5774174
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC396074/
Abstract

Total RNA and DNA of mimosa epicotyl tissues were extracted and the RNA fractionated into specific soluble RNAs (sRNAs) at different times during late germination. Epicotyls collected at each time contained qualitatively comparable meristematic and developing tissues, while mature tissues increased. Quantitative ratios of total RNA to DNA and total sRNAs to approximated ribosomal RNA (rRNA) varied consistently during development. Terminal nucleosides of sRNA did not vary in any consistent pattern through development. On the other hand, regular changes in quantitative ratios of specific sRNA groups were observed during development.

摘要

提取含羞草上胚轴组织的总RNA和DNA，并在种子萌发后期的不同时间将RNA分级分离成特定的可溶性RNA（sRNA）。每次收集的上胚轴含有性质相当的分生组织和发育中的组织，同时成熟组织增加。在发育过程中，总RNA与DNA以及总sRNA与近似核糖体RNA（rRNA）的定量比率持续变化。在整个发育过程中，sRNA的末端核苷没有呈现出任何一致的变化模式。另一方面，在发育过程中观察到特定sRNA组定量比率的规律性变化。

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Biochem J. 1951 May;48(5):584-90. doi: 10.1042/bj0480584.
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Anal Biochem. 1960 Jun;1:66-77. doi: 10.1016/0003-2697(60)90020-8.
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Proc Natl Acad Sci U S A. 1965 Mar;53(3):549-56. doi: 10.1073/pnas.53.3.549.
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