Williamson A R
Biochem J. 1969 Feb;111(4):515-20. doi: 10.1042/bj1110515.
The attachment of polyuridylic acid to reticulocyte ribosomes was studied by using polyadenylic acid, which inhibits the attachment reaction only, while permitting translation of polyuridylic acid bound to ribosomes. After addition of polyadenylic acid the amount of polyphenylalanine synthesized under standard conditions was taken as a measure of the bound polyuridylic acid. In this way certain parameters of the attachment reaction and the subsequent translation of attached polyuridylic acid were defined: (1) polyuridylic acid-ribosome interaction at 37 degrees requires only Mg(2+) at an optimum concentration of 8mm; (2) K(+) (required for translation) is a non-competitive inhibitor of the attachment reaction; (3) optimum polyphenylalanine synthesis directed by attached polyuridylic acid occurs at 5mm-Mg(2+) concentration; (4) from kinetic studies single ribosomes appear to participate in the attachment reaction.
通过使用聚腺苷酸研究了聚尿苷酸与网织红细胞核糖体的结合，聚腺苷酸仅抑制结合反应，同时允许与核糖体结合的聚尿苷酸进行翻译。加入聚腺苷酸后，在标准条件下合成的聚苯丙氨酸量被用作结合的聚尿苷酸的量度。通过这种方式确定了结合反应以及随后结合的聚尿苷酸翻译的某些参数：（1）37℃下聚尿苷酸与核糖体的相互作用仅需要最佳浓度为8mM的Mg(2+)；（2）翻译所需的K(+)是结合反应的非竞争性抑制剂；（3）结合的聚尿苷酸指导的最佳聚苯丙氨酸合成发生在5mM-Mg(2+)浓度下；（4）动力学研究表明单个核糖体似乎参与了结合反应。
