体内氨基甲酸乙酯与核酸的反应。
Reaction of urethane with nucleic acids in vivo.
作者信息
Boyland E, Williams K
出版信息
Biochem J. 1969 Jan;111(1):121-7. doi: 10.1042/bj1110121.
Abstract
- [1-(14)C]Ethyl carbamate, ethyl [carboxy-(14)C]carbamate, [1-(14)C]ethanol and sodium hydrogen [(14)C]carbonate were injected intraperitoneally into C57 mice, and nucleic acids and proteins were separated from the liver and lungs with phenol as described by Kirby (1956). 2. Chromatographic analysis of the hydrolytic products of the urethane-labelled RNA showed the presence of a single radioactive compound differing in behaviour from the major pyrimidine nucleotides and purines. 3. The products from RNA labelled by [1-(14)C]ethyl carbamate or ethyl [carboxy-(14)C]carbamate appeared chromatographically identical but could not be detected in the RNA of mice given [1-(14)C]ethanol or sodium hydrogen [(14)C]-carbonate. 4. The labelled product appeared to be the ethyl ester of cytosine-5-carboxylic acid formed by the reaction of urethane with RNA in vivo. 5. A direct reaction between labelled urethane or the labelled metabolite of urethane, [1-(3)H]-ethyl N-hydroxycarbamate, and RNA was not detected.
摘要
- 将[1-(14)C]氨基甲酸乙酯、乙基[羧基-(14)C]氨基甲酸酯、[1-(14)C]乙醇和碳酸氢钠[(14)C]腹腔注射到C57小鼠体内，然后按照Kirby（1956年）所述方法，用苯酚从肝脏和肺中分离核酸和蛋白质。2. 对氨基甲酸乙酯标记的RNA水解产物进行色谱分析，结果显示存在一种单一放射性化合物，其行为与主要嘧啶核苷酸和嘌呤不同。3. [1-(14)C]氨基甲酸乙酯或乙基[羧基-(14)C]氨基甲酸酯标记的RNA产物在色谱上显示相同，但在给予[1-(14)C]乙醇或碳酸氢钠[(14)C]的小鼠RNA中未检测到。4. 标记产物似乎是氨基甲酸乙酯与体内RNA反应形成的胞嘧啶-5-羧酸乙酯。5. 未检测到标记的氨基甲酸乙酯或其标记代谢产物[1-(3)H]-N-羟基氨基甲酸乙酯与RNA之间的直接反应。
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