Bray J P, Bass J A
Appl Microbiol. 1969 Feb;17(2):232-6. doi: 10.1128/am.17.2.232-236.1969.
The complex of enzymes elaborated by a strain of Streptomyces albus was separated by Sephadex G-75 column chromatography. Two major and one minor proteolytic peaks and one streptolytic peak were obtained. The streptolytic fraction alone did not cause lysis of the cells, but was highly effective in the presence of small amounts of trypsin or of the proteolytic components of the enzyme complex. Treatment of cells with the purified streptolytic fraction effected release of group carbohydrate and of a trypsin and pepsin-resistant protein antigen which reacted with type-specific antisera.
一株白链霉菌所产生的复合酶通过葡聚糖G - 75柱层析进行分离。得到了两个主要的和一个次要的蛋白水解峰以及一个溶菌峰。单独的溶菌组分不会导致细胞裂解，但在存在少量胰蛋白酶或酶复合物的蛋白水解成分时非常有效。用纯化的溶菌组分处理细胞会使类碳水化合物以及一种与型特异性抗血清反应的、对胰蛋白酶和胃蛋白酶有抗性的蛋白质抗原释放出来。
