Yasukouchi T, Fujine M, Kohn S, Sakurama S, Morioka T
Thromb Haemost. 1977 Jun 30;37(3):456-63.
The optimal conditions for the measurement of the fibrinolytic factors of plasma were examined using human and bovine plasminogen-rich fibrinogen or plasminogen-free fibrinogen as the substrates using the one dimensional diffusion method. The results were as follows: 1. There was no essential difference found between using human or bovine fibrinogen. 2. The levels of proactivator-plasminogen and plasminogen could be measured while using either plasminogen-rich or plasminogen-free fibrinogen. But, in using the latter, the proactivator-plasminogen level could not be measured, if a final concentration of more than 2,000 Christensen units of streptokinase were employed. 3. When using plasminogen-rich fibrinogen, anti-plasmin(s) and anti-activator(s) could be measured while using urokinase and plasmin, but not while using streptokinase. However, further study should be given to the measurement of the inhibitors, when using plasminogen-free fibrinogen.
采用一维扩散法，以富含人及牛纤溶酶原的纤维蛋白原或无纤溶酶原的纤维蛋白原为底物，研究血浆纤溶因子测定的最佳条件。结果如下：1. 使用人或牛纤维蛋白原之间未发现本质差异。2. 使用富含纤溶酶原或无纤溶酶原的纤维蛋白原均可测定前激活物-纤溶酶原和纤溶酶原水平。但是，使用后者时，如果链激酶的终浓度超过2000克里斯滕森单位，则无法测定前激活物-纤溶酶原水平。3. 使用富含纤溶酶原的纤维蛋白原时，使用尿激酶和纤溶酶可测定抗纤溶酶和抗激活物，但使用链激酶时则不能。然而，使用无纤溶酶原的纤维蛋白原时，对抑制剂的测定应进一步研究。
