Mohanty G C, Rajya B S
Vet Pathol. 1977 Jul;14(4):420-5. doi: 10.1177/030098587701400414.
Canine venereal tumor cells were grown in monolayer and tumor tissue fragments were maintained in vitro for 56 days in medium-199. Attachment and cell replication were evident within 17 hours and monolayer was obtained by the tenth day. Growth at first comprised mainly round cells with abundance of cytoplasm and vesicular nuclei, and a few spindle-shaped cells. Later cells became more elongated and like fibroblasts. Cell degeneration started by the 17th day and most were degenerated by the 24th day. Necrosis and depletion of cell population were prominent in tumor explants during the first 2 weeks. Distinct cell multiplication was evident by the 21st day and tumor fragments were repopulated with cells resembling the original tumor by the 56th day. Two distinct morphological cell types were seen: small cells with vesicular, round to oval nuclei and acidophilic cytoplasm; and the large cells with large hyperchromatic nuclei and acidophilic cytoplasm.
犬传染性肿瘤细胞以单层培养，肿瘤组织碎片在199培养基中体外培养56天。17小时内可见细胞附着和复制，到第10天形成单层。最初生长的主要是圆形细胞，细胞质丰富，核呈泡状，还有一些梭形细胞。后来细胞变得更加细长，类似成纤维细胞。到第17天开始出现细胞退化，到第24天大多数细胞已退化。在最初的2周内，肿瘤外植体中细胞坏死和数量减少很明显。到第21天明显可见细胞增殖，到第56天肿瘤碎片重新被类似原始肿瘤的细胞占据。可见两种不同形态的细胞类型：小细胞，核呈泡状，圆形至椭圆形，细胞质嗜酸性；大细胞，核大且染色质深，细胞质嗜酸性。
