葡萄球菌凝固酶测定的标准化程序。
Standardized procedure for determination of staphylocoagulase.
作者信息
Wegrzynowicz Z, Jeljaszewicz J, Lipinski B
出版信息
Appl Microbiol. 1969 Apr;17(4):556-8. doi: 10.1128/am.17.4.556-558.1969.
Abstract
A simple standardized procedure for the determination of staphylocoagulase, based on thrombin clotting time measurement with euglobulin, has been developed. A standard euglobulin solution containing 0.25% of clottable protein, 0.01 M epsilon-aminocaproic acid, and 20 units of heparin per ml is employed. One unit of staphylocoagulase is defined as the amount of the enzyme which clots this standard euglobulin solution in 25 sec.
摘要
已开发出一种基于用优球蛋白测定凝血酶凝结时间来测定葡萄球菌凝固酶的简单标准化程序。使用每毫升含有0.25%可凝蛋白、0.01Mε-氨基己酸和20单位肝素的标准优球蛋白溶液。1单位葡萄球菌凝固酶定义为在25秒内使该标准优球蛋白溶液凝固的酶量。
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