Stutzenberger F J, Clemente C L, Vadehra D V
J Bacteriol. 1966 Oct;92(4):1005-9. doi: 10.1128/jb.92.4.1005-1009.1966.
Stutzenberger, Fred J. (Michigan State University, East Lansing), Charles L. San Clemente, and Dharam V. Vadehra. Nephelometric assay of staphylococcal coagulase. J. Bacteriol. 92:1005-1009. 1966.-Clotting of fibrinogen by staphylococcal coagulase was accompanied by an increase in light scattering; this property was used as a basis for a new nephelometric method. Reaction rates, which were now easily and precisely measured, were found to be directly proportional to coagulase concentration, when optimal conditions were maintained. These conditions included pH and concentrations of fibrinogen, coagulase-reacting factor, and sodium chloride in the reaction mixture. A standardized procedure for the assay is outlined, and a unit for the expression of activity is proposed.
施图岑贝格尔，弗雷德·J.（密歇根州立大学，东兰辛），查尔斯·L. 圣克莱门特，以及达拉姆·V. 瓦德赫拉。葡萄球菌凝固酶的比浊测定法。《细菌学杂志》92:1005 - 1009。1966年。——葡萄球菌凝固酶使纤维蛋白原凝固的过程伴随着光散射的增加；这一特性被用作一种新的比浊法的基础。当保持最佳条件时，发现现在易于精确测量的反应速率与凝固酶浓度成正比。这些条件包括反应混合物中的pH值以及纤维蛋白原、凝固酶反应因子和氯化钠的浓度。概述了该测定的标准化程序，并提出了一种活性表达单位。
