Van de Water T R
Arch Otorhinolaryngol. 1977 Aug 26;217(3):297-311. doi: 10.1007/BF00465547.
Preliminary observations of the effect of surgical excision of the endolymphatic duct and sac anlage in normal mouse embryo inner ear embryogenesis "in vitro" are reported. Otocysts of twelfth and thirteenth gestation day mouse embryos were grown in organ culture for 9 and 8 days respectively. The organ culture specimens of each gestational age group were further divided into otocysts with intact endolymphatic duct and sac anlages and otocysts that had their endolymphatic duct and sac anlages surgically excised prior to explantation. The resultant histological quantification of inner ear embryogenesis did not reveal any significant differences in morphogenesis, histodifferentiation of inner ear sensory structures or presence of endolymphatic hydrops in any of the organ culture specimens. It was concluded that in the system studied the absence of the endolymphatic duct and sac anlage did not have an effect on the organogenesis of the inner ear with the exception of the resultant lack of development of an endolymphatic duct and sac.
报告了对内淋巴囊管和囊原基进行手术切除对正常小鼠胚胎内耳胚胎发生“体外”影响的初步观察结果。妊娠第12天和第13天小鼠胚胎的听泡分别在器官培养中培养9天和8天。每个胎龄组的器官培养标本进一步分为内淋巴囊管和囊原基完整的听泡以及在植入前手术切除了内淋巴囊管和囊原基的听泡。内耳胚胎发生的组织学定量分析结果显示，任何器官培养标本在内耳形态发生、内耳感觉结构的组织分化或内淋巴积水的存在方面均未发现任何显著差异。得出的结论是，在所研究的系统中，除了导致内淋巴囊管和囊未发育外，内淋巴囊管和囊原基的缺失对内耳器官发生没有影响。
