Trench R K, Greene R W, Bystrom B G
J Cell Biol. 1969 Aug;42(2):404-17. doi: 10.1083/jcb.42.2.404.
The marine gastropod molluscs Tridachia crispata, Tridachiella diomedea, and Placobranchus ianthobapsus (Sacoglossa, Opisthobranchia) possess free functional chloroplasts within the cells of the digestive diverticula, as determined by observations on ultrastructure, pigment analyses, and experiments on photosynthetic capacity. In the light, the chloroplasts incorporate H(14)CO(3) (-)in situ. Reduced radiocarbon is translocated to various chloroplast-free tissues in the animals. The slugs feed on siphonaceous algae from which the chloroplasts are derived. Pigments from the slugs and from known siphonaceous algae, when separated chromatographically and compared, showed similar components. Absorption spectra of extracts of slugs and algae were very similar. The larvae of the slugs are pigment-free up to the post-veliger stage, suggesting that chloroplasts are acquired de novo. with each new generation.
通过超微结构观察、色素分析以及光合能力实验确定，海洋腹足纲软体动物脆壳海兔（Tridachia crispata）、迪氏海兔（Tridachiella diomedea）和伊氏叶鳃海牛（Placobranchus ianthobapsus，囊舌目，后鳃亚纲）在消化盲囊细胞内拥有游离的功能性叶绿体。在光照条件下，叶绿体在原位吸收H(14)CO(3) (-)。还原的放射性碳被转运到动物体内各种不含叶绿体的组织中。这些海蛞蝓以叶绿体来源的管叶藻为食。对海蛞蝓和已知管叶藻的色素进行色谱分离并比较后，发现它们的成分相似。海蛞蝓和藻类提取物的吸收光谱非常相似。海蛞蝓的幼虫在面盘幼虫后期之前是无色素的，这表明叶绿体是每一代重新获得的。
