在血液凝固过程中，通过七鳃鳗内源性因子XIII和人因子XIII的作用，比较七鳃鳗纤维蛋白原和纤维蛋白的交联模式。

Comparison of the cross-linking patterns of lamprey fibrinogen and fibrin by the action of the intrinsic lamprey factor XIII and human factor XIII during the process of blood coagulation.

作者信息

Murtaugh P A

出版信息

Thromb Haemost. 1977 Aug 31;38(2):429-37.

Abstract

Intrinsic lamprey factor XIII cross-links the gamma chain of lamprey fibrin (50,000 daltons) to the gamma-dimer (100,000 daltons). The alpha-chain (110,000 daltons) is cross-linked very slowly to alpha-dimer (210,000 daltons) and alpha-trimer (330,000 daltons). In contrast, human factor XIII, when added in combination with intrinsic lamprey factor XIII, cross-links the alpha-chain of lamprey fibrin to a high molecular weight polymer, and any remaining gamma-chain is also cross-linked to a polymer. However, the gamma-chain that has previously cross-linked to the gamma-dimer by the intrinsic lamprey factor XIII remains as a gamma-dimer. Factor XIII-free lamprey fibrin cross-links all its subunits (alpha, beta, gamma) to high molecular weight polymers when human factor XIII is added. In contrast to human and bovine fibrin where alpha-chain cross-linking in the process of blood coagulation commences when all of the gamma-chain has cross-linked, the lamprey alpha-chain will begin to cross-link when approximately half of the gamma-chain has cross-linked to the gamma-dimer.

摘要

七鳃鳗内源性因子XIII将七鳃鳗纤维蛋白的γ链（50,000道尔顿）交联至γ二聚体（100,000道尔顿）。α链（110,000道尔顿）与α二聚体（210,000道尔顿）和α三聚体（330,000道尔顿）的交联非常缓慢。相比之下，人因子XIII与七鳃鳗内源性因子XIII联合添加时，会将七鳃鳗纤维蛋白的α链交联至高分子量聚合物，任何剩余的γ链也会交联至聚合物。然而，先前已通过七鳃鳗内源性因子XIII交联至γ二聚体的γ链仍为γ二聚体。当添加人因子XIII时，不含因子XIII的七鳃鳗纤维蛋白会将其所有亚基（α、β、γ）交联至高分子量聚合物。与人和牛纤维蛋白不同，在人和牛纤维蛋白中，当所有γ链都已交联时，血液凝固过程中的α链交联才开始，而七鳃鳗α链在大约一半的γ链交联至γ二聚体时就会开始交联。