Garrett J R, Parsons P A
Histochem J. 1976 Mar;8(2):177-89. doi: 10.1007/BF01007167.
Horseradish peroxidase (HRP) has been used as a tracer to study movements of solutions injected retrogradely via the duct of submandibular glands in rabbits. 0.1 ml of solution was injected either manually or by a constant hydrostatic pressure, and the subsequent distribution of HRP in the gland and duct at different times after injection has been examined histochemically at light and electron microscopical levels. Shortly after the injections, strong interstitial staining for peroxidase resulted from passage between acinar cells. Some sites of cellular uptake were observed and staining occurred in some ductal cells even when the duct had been cut at the hilum to minimize pressure effects. It is not known whether this diffuse uptake represents a physiological or pathological phenomenon. Some interstitial activity still remained 24 hr after injection but had disappeared by 48 hr. Inflammatory cells first appeared in the gland about 4hr after the injection and slowly increased up to about 24 hr after injection. The results indiate that the HRP reaches the interstices of the gland principally by penetration between acinar cells, and that the junctional complexes between striated duct cells appear to be more resistant to disruption by luminal pressures.
辣根过氧化物酶（HRP）已被用作示踪剂，以研究经兔下颌下腺导管逆行注射的溶液的流动情况。通过手动或恒定静水压力注射0.1毫升溶液，并在注射后不同时间，在光学和电子显微镜水平上通过组织化学方法检查HRP在腺体和导管中的后续分布。注射后不久，腺泡细胞之间的通道导致间质中出现强烈的过氧化物酶染色。观察到一些细胞摄取部位，即使在腺门处切断导管以尽量减少压力影响时，一些导管细胞中也出现了染色。尚不清楚这种弥漫性摄取是代表生理现象还是病理现象。注射后24小时仍有一些间质活性，但在48小时时已消失。注射后约4小时，炎症细胞首次出现在腺体中，并在注射后约24小时缓慢增加。结果表明，HRP主要通过腺泡细胞之间的渗透到达腺体间质，并且纹状管细胞之间的连接复合体似乎对腔内压力的破坏更具抵抗力。
