Daneholt B, Edström J E
J Cell Biol. 1969 May;41(2):620-4. doi: 10.1083/jcb.41.2.620.
The base composition of DNA was determined for individual chromosomes from the dipteran Chironomus tentans and for each one of six different segments of one of the chromosomes. The isolations were carried out by micromanipulation and the DNA purines were first extracted from the isolated components and afterwards separated by means of microelectrophoresis on a cellulose fiber. It was found that DNA from this material has an unusual composition corresponding to a guanine + cytosine content of about 30%. This composition was not a function of the polytenic condition but was also found for DNA from testis tissue. Furthermore Drosophila has a more traditional base composition for the bulk of DNA. Statistically significant variations in base data were found between whole chromosomes as well as between the segments from one of the chromosomes.
测定了双翅目昆虫摇蚊的单个染色体以及其中一条染色体的六个不同区段中每个区段的DNA碱基组成。通过显微操作进行分离,首先从分离出的组分中提取DNA嘌呤,然后在纤维素纤维上通过微电泳进行分离。发现这种材料的DNA具有不寻常的组成,鸟嘌呤+胞嘧啶含量约为30%。这种组成不是多线状态的函数,在睾丸组织的DNA中也发现了这种情况。此外,果蝇大部分DNA的碱基组成更传统。在整条染色体之间以及一条染色体的各个区段之间,碱基数据存在统计学上显著的差异。