el-Din M Z, el-Shazly K
Appl Microbiol. 1969 Jun;17(6):801-4. doi: 10.1128/am.17.6.801-804.1969.
The method of el-Shazly and Hungate for measuring gas production in rumen contents was slightly modified and used throughout this investigation. The variation in the fermentation rates due to samples collected separately from a sheep fed on hay was less than 2%. When samples obtained through a stomach tube were compared with samples collected through the rumen fistula, the variation was about 3%. The rates of gas and acid production were approximately similar in samples obtained from the rumen at the same time when no sodium bicarbonate was added. During in vitro incubation of whole ruminal contents, there was a highly significant correlation between the net growth rate values (obtained by using fermentation capacity as an index) and the change in concentration of viable rumen bacteria or total ciliate protozoa.
在整个研究过程中，对el-Shazly和Hungate测量瘤胃内容物气体产生的方法进行了轻微修改并加以应用。从以干草为食的绵羊身上分别采集的样本，其发酵速率的变化小于2%。当将通过胃管获得的样本与通过瘤胃瘘管采集的样本进行比较时，变化约为3%。在不添加碳酸氢钠的情况下，从瘤胃同时获取的样本中，气体和酸的产生速率大致相似。在整个瘤胃内容物的体外培养过程中，（以发酵能力为指标获得的）净生长速率值与瘤胃活菌或总纤毛虫原生动物浓度的变化之间存在高度显著的相关性。
